Plasmid
pEryU

Part:BBa_K4383044:Design

Designed by: Junxiao Zhang   Group: iGEM22_SCU-China   (2022-10-10)


pEryU


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 4355
    Illegal NheI site found at 4378
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 5998
    Illegal AgeI site found at 880
    Illegal AgeI site found at 1324
    Illegal AgeI site found at 2166
    Illegal AgeI site found at 3347
    Illegal AgeI site found at 5414
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 5501


Design Notes

Previous studies have identified the erythritol-utilizing gene clusters in the genome of B. abortus<i/> and confirmed that the five enzymes expressed could achieve the utilization from erythritol to D-erythrose-4-P (Barbier T et al., 2014). Therefore, we would like to introduce this pathway into E. coli. Considering the known functions of the enzymes in the erythritol utilizing gene clusters, we constructed the pJ23114-eryA-eryB-eryC and pJ23114-eryR-eryH-eryI plasmid, and then got an engineered bacteria of eryABCRHI.

Source

<i>Brucella abortus

References

Barbier T, Collard F, Zúñiga-Ripa A, Moriyón I, Godard T, Becker J, Wittmann C, Van Schaftingen E, Letesson JJ. Erythritol feeds the pentose phosphate pathway via three new isomerases leading to D-erythrose-4-phosphate in Brucella. Proc Natl Acad Sci U S A. 2014 Dec 16;111(50):17815-20. doi: 10.1073/pnas.1414622111. Epub 2014 Dec 2.